Western blot analysis of cell lysates of HeLa (40 ug) were resolved by SDS - PAGE , transferred to NC membrane and probed with IRF3 monoclonal antibody , clone 3F10 (1 : 1000) (Cat # MAB1103). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
IRF3 encodes interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. [provided by RefSeq