IRF3 monoclonal antibody, clone 3F10
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of HeLa cell lysates.
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The stained with IRF3 monoclonal antibody, clone 3F10 (1:500) with Texas-Red and Hoechst 33342 (Blue) for nucleus staining.
Flow Cytometry
Flow cytometry analysis of IRF3 in MCF7 cells. The cell was stained with MAB1103 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant IRF3.
Immunogen
Recombinant protein corresponding to amino acids 108-166 of human IRF3.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HeLa cell lysates.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The stained with IRF3 monoclonal antibody, clone 3F10 (1:500) with Texas-Red and Hoechst 33342 (Blue) for nucleus staining.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometry analysis of IRF3 in MCF7 cells. The cell was stained with MAB1103 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray). -
Gene Info — IRF3
Entrez GeneID
3661GeneBank Accession#
NM_001571Protein Accession#
NP_001562Gene Name
IRF3
Gene Alias
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Gene Description
interferon regulatory factor 3
Omim ID
603734Gene Ontology
HyperlinkGene Summary
IRF3 encodes interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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Disease
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Publication Reference
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Multiple NF-kappaB and IFN regulatory factor family transcription factors regulate CCL19 gene expression in human monocyte-derived dendritic cells.
Pietila TE, Veckman V, Lehtonen A, Lin R, Hiscott J, Julkunen I.
Journal of Immunology 2007 Jan; 178(1):253.
Application：WB-Ce, Human, Human monocyte-derived dendritic cells.
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Interferon regulatory factor 3 is necessary for induction of antiviral genes during human cytomegalovirus infection.
DeFilippis VR, Robinson B, Keck TM, Hansen SG, Nelson JA, Fruh KJ.
Journal of Virology 2006 Jan; 80(2):1032.
Application：IF, WB-Tr, Human, Foreskin fibroblasts, HF-Control siRNA, HF-IRF3 siRNA cells.
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Multiple NF-kappaB and IFN regulatory factor family transcription factors regulate CCL19 gene expression in human monocyte-derived dendritic cells.
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