IRF3 (phospho S386) recombinant monoclonal antibody, clone 4H10
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate (treated with Calyculin A 100 nM/60 mins) and Lane 2: Hela whole cell lysate (not treated) with IRF3 (phospho S386) recombinant monoclonal antibody, clone 4H10 (Cat # RAB04238).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human IRF3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding S386 of human IRF3.
Theoretical MW (kDa)
Calculated MW: 47 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate (treated with Calyculin A 100 nM/60 mins) and Lane 2: Hela whole cell lysate (not treated) with IRF3 (phospho S386) recombinant monoclonal antibody, clone 4H10 (Cat # RAB04238).Enzyme-linked Immunoabsorbent Assay
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Gene Info — IRF3
Entrez GeneID
3661Protein Accession#
Q14653Gene Name
IRF3
Gene Alias
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Gene Description
interferon regulatory factor 3
Omim ID
603734Gene Ontology
HyperlinkGene Summary
IRF3 encodes interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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Disease
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