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Last updated: 2020/1/19
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HSPA1A monoclonal antibody, clone 4E11 

  • Catalog # : MAB1094
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against full length recombinant HSPA1A.
  • Immunogen:
  • Recombinant protein corresponding to full length human HSPA1A.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G affinity chromatography
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • ELISA
    Immunocytochemistry/Immunofluorescence
    Immunohistochemistry (1:100-1:200)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at 4°C for 1 week. For long term storage, store at -20°C to -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of (1) 293T cell lysate, and (2) HeLa cell lysate. The cell lysates (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with HSPA1A monoclonal antibody , clone 4E11 (Cat # MAB1094). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Paraffin embedded sections of human eccrine gland were incubated with HSPA1A monoclonal antibody , clone 4E11 (Cat # MAB1094) (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of HSPA1A in A549 cells line, stained with DAPI (Blue) for nucleus staining and HSPA1A monoclonal antibody , clone 4E11 (Cat # MAB1094) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
  • Enzyme-linked Immunoabsorbent Assay
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Entrez GeneID:
  • 3303
  • Gene Name:
  • HSPA1A
  • Gene Alias:
  • FLJ54303,FLJ54370,FLJ54392,FLJ54408,FLJ75127,HSP70-1,HSP70-1A,HSP70I,HSP72,HSPA1,HSPA1B
  • Gene Description:
  • heat shock 70kDa protein 1A
  • Gene Summary:
  • This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000029287,dnaK-type molecular chaperone HSP70-1,heat shock 70kD protein 1A,heat shock 70kDa protein 1B,heat shock-induced protein
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