HSPA1A monoclonal antibody, clone 4E11
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: 293T cell lysate; Lane 2: HeLa cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of human eccrine gland were incubated with HSPA1A monoclonal antibody , clone 4E7 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).
Immunofluorescence
Immunofluorescent staining of A549 cells line, stained with DAPI (Blue) for nucleus staining and HSPA1A monoclonal antibody, clone 4E7 with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant HSPA1A.
Immunogen
Recombinant protein corresponding to full length human HSPA1A.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Recommend Usage
ELISA
Immunocytochemistry
Immunofluorescence
Immunohistochemistry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: 293T cell lysate; Lane 2: HeLa cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of human eccrine gland were incubated with HSPA1A monoclonal antibody , clone 4E7 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).Immunofluorescence
Immunofluorescent staining of A549 cells line, stained with DAPI (Blue) for nucleus staining and HSPA1A monoclonal antibody, clone 4E7 with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).Enzyme-linked Immunoabsorbent Assay
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Gene Info — HSPA1A
Entrez GeneID
3303GeneBank Accession#
NM_005345Protein Accession#
NP_005336Gene Name
HSPA1A
Gene Alias
FLJ54303, FLJ54370, FLJ54392, FLJ54408, FLJ75127, HSP70-1, HSP70-1A, HSP70I, HSP72, HSPA1, HSPA1B
Gene Description
heat shock 70kDa protein 1A
Omim ID
140550Gene Ontology
HyperlinkGene Summary
This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq
Other Designations
OTTHUMP00000029287|dnaK-type molecular chaperone HSP70-1|heat shock 70kD protein 1A|heat shock 70kDa protein 1B|heat shock-induced protein
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Interactome
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Pathway
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Disease
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Publication Reference
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Proteomic study identified HSP 70kDa protein 1A as a possible therapeutic target, in combination with histone deacetylase inhibitors, for lymphoid neoplasms.
Fujii K, Suzuki N, Ikeda K, Hamada T, Yamamoto T, Kondo T, Iwatsuki K.
Journal of Proteomics 2012 Feb; 75(4):1401.
Application：WB-Ce, Human, Lymphoid neoplasm cell.
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The chaperone function of hsp70 is required for protection against stress-induced apoptosis.
Mosser DD, Caron AW, Bourget L, Meriin AB, Sherman MY, Morimoto RI, Massie B.
Molecular and Cellular Biology 2000 Oct; 20(19):7146.
Application：Flow Cyt, WB, Human, PEER cells.
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Protein folding in the cell.
Gething MJ, Sambrook J.
Nature 1992 Jan; 355(6355):33.
Application：WB-Ce, WB-Re, WB-Tr, Bacteria, Firefly, Human, Mammalian cells, Recombinant proteins.
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Involvement of ATP in the nuclear and nucleolar functions of the 70 kd heat shock protein.
Lewis MJ, Pelham HR.
The EMBO Journal 1985 Dec; 4(12):3137.
Application：IF, WB-Ce, WB-Tr, Human, Monkey, Rat, COS-7, HEK 293, HeLa, rat-1 cells.
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Proteomic study identified HSP 70kDa protein 1A as a possible therapeutic target, in combination with histone deacetylase inhibitors, for lymphoid neoplasms.
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