HSP70 monoclonal antibody, clone 3G10
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of pig skeletal muscle.
Western Blot
Western Blot analysis of Line 1: HeLa cell and Line 2: mouse brain tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lung carcicnoma.
Immunofluorescence
Immunofluorescence staining of HeLa cell line with antibody (Left), DAPI (Middle) and merge (Right).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of human HSP70.
Immunogen
A synthetic peptide corresponding to N-terminus of human HSP70.
Host
Mouse
Reactivity
Human, Mouse, Pig
Form
Liquid
Purification
Affinity purification
Isotype
IgG1
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunofluorescence (1:100-1:200)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 0.5% BSA, 0.02% sodium azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of pig skeletal muscle.Western Blot
Western Blot analysis of Line 1: HeLa cell and Line 2: mouse brain tissue.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lung carcicnoma.Immunofluorescence
Immunofluorescence staining of HeLa cell line with antibody (Left), DAPI (Middle) and merge (Right). -
Gene Info — HSPA1A
Entrez GeneID
3303Protein Accession#
P34931Gene Name
HSPA1A
Gene Alias
FLJ54303, FLJ54370, FLJ54392, FLJ54408, FLJ75127, HSP70-1, HSP70-1A, HSP70I, HSP72, HSPA1, HSPA1B
Gene Description
heat shock 70kDa protein 1A
Omim ID
140550Gene Ontology
HyperlinkGene Summary
This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq
Other Designations
OTTHUMP00000029287|dnaK-type molecular chaperone HSP70-1|heat shock 70kD protein 1A|heat shock 70kDa protein 1B|heat shock-induced protein
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Gene Info — HSPA1B
Entrez GeneID
3304Protein Accession#
P34931Gene Name
HSPA1B
Gene Alias
FLJ54328, HSP70-1B, HSP70-2, HSPA1A
Gene Description
heat shock 70kDa protein 1B
Omim ID
603012Gene Ontology
HyperlinkGene Summary
This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq
Other Designations
OTTHUMP00000029288|heat shock 70kD protein 1B|heat shock 70kDa protein 1A
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Gene Info — HSPA1L
Entrez GeneID
3305Protein Accession#
P34931Gene Name
HSPA1L
Gene Alias
HSP70-1L, HSP70-HOM, HSP70T, hum70t
Gene Description
heat shock 70kDa protein 1-like
Omim ID
140559Gene Ontology
HyperlinkGene Summary
This gene encodes a 70kDa heat shock protein. In conjunction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which also encode isoforms of the 70kDa heat shock protein. [provided by RefSeq
Other Designations
OTTHUMP00000029295|heat shock 10kDa protein 1-like|heat shock 70kD protein-like 1
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Interactome
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Pathway
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Disease
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