Products

Western Blot (Cell lysate)

Western blot analysis of Lane1:MCF-7 whole cell lysate Lane2:HCT116 whole cell lysate with HSPA1B recombinant monoclonal antibody (Cat # RAB02656) at 1:1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSPA1B recombinant monoclonal antibody (Cat # RAB02656). Counter stained with hematoxylin.

Immunocytochemistry

Immunocytochemical staining of HeLa cells using HSPA1B recombinant monoclonal antibody (Cat # RAB02656)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.

Immunofluorescence

Flow Cytometry

Flow cytometric analysis of Hela cells with HSPA1B recombinant monoclonal antibody (Cat # RAB02656) at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Entrez GeneID

3304

Protein Accession#

P0DMV9

Gene Name

HSPA1B

Gene Alias

FLJ54328, HSP70-1B, HSP70-2, HSPA1A

Gene Description

heat shock 70kDa protein 1B

Omim ID

603012

Gene Ontology

Hyperlink

Gene Summary

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq

Other Designations

OTTHUMP00000029288|heat shock 70kD protein 1B|heat shock 70kDa protein 1A