ISG15 monoclonal antibody, clone 3E5
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HeLa cell lysate; Lane 2: MCF7 cell lysate.
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with ISG15 monoclonal antibody, clone 3E5 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant ISG15.
Immunogen
Recombinant protein corresponding to amino acids 1-157 of human ISG15.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Isotype
IgG1, kappa
Recommend Usage
ELISA
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HeLa cell lysate; Lane 2: MCF7 cell lysate.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with ISG15 monoclonal antibody, clone 3E5 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — ISG15
Entrez GeneID
9636GeneBank Accession#
NM_005101Protein Accession#
NP_005092Gene Name
ISG15
Gene Alias
G1P2, IFI15, UCRP
Gene Description
ISG15 ubiquitin-like modifier
Omim ID
147571Gene Ontology
HyperlinkGene Summary
ISG15 is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha (IFNA; MIM 147660) and -beta (IFNB; MIM 147640) (Zhao et al., 2005 [PubMed 16009940]).[supplied by OMIM
Other Designations
OTTHUMP00000038464|interferon, alpha-inducible protein (clone IFI-15K)|interferon-induced 17-kDa/15-kDa protein|interferon-stimulated protein, 15 kDa|ubiquitin cross-reactive protein
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Interactome
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Disease
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