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Last updated: 2020/5/24
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ISG15 monoclonal antibody, clone 3E5 

  • Catalog # : MAB1090
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant ISG15.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 1-157 of human ISG15.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • ELISA
    Immunocytochemistry
    Immunofluorescence
    Western Blot
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: HeLa cell lysate; Lane 2: MCF7 cell lysate.
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of HeLa cells. The cell was stained with ISG15 monoclonal antibody, clone 3E5 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
  • Enzyme-linked Immunoabsorbent Assay
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunocytochemistry
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Entrez GeneID:
  • 9636
  • Gene Name:
  • ISG15
  • Gene Alias:
  • G1P2,IFI15,UCRP
  • Gene Description:
  • ISG15 ubiquitin-like modifier
  • Gene Summary:
  • ISG15 is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha (IFNA; MIM 147660) and -beta (IFNB; MIM 147640) (Zhao et al., 2005 [PubMed 16009940]).[supplied by OMIM
  • Other Designations:
  • OTTHUMP00000038464,interferon, alpha-inducible protein (clone IFI-15K),interferon-induced 17-kDa/15-kDa protein,interferon-stimulated protein, 15 kDa,ubiquitin cross-reactive protein
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