Western blot analysis of HeLa cell lysate (each 50 ug) was resolved by SDS - PAGE , transferred to PVDF membrane and probed with ISG15 monoclonal antibody , clone 3E5 (1 : 1000) (Cat # MAB1090). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
ISG15 is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha (IFNA; MIM 147660) and -beta (IFNB; MIM 147640) (Zhao et al., 2005 [PubMed 16009940]).[supplied by OMIM
OTTHUMP00000038464,interferon, alpha-inducible protein (clone IFI-15K),interferon-induced 17-kDa/15-kDa protein,interferon-stimulated protein, 15 kDa,ubiquitin cross-reactive protein