NME1 monoclonal antibody, clone 4B2

Catalog # MAB10833

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Quantity

Size:100 uL
Price: USD $ 531.00
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Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Images
Western Blot (Transfected lysate)
Application

Western Blot (Transfected lysate)

Western blot analysis using NME1 monoclonal antibody, clone 4B2 (Cat # MAB10833) against NME1-hIgGFc transfected HEK293 cell lysate.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunohistochemical analysis of paraffin-embedded human placenta tissues using NME1 monoclonal antibody, clone 4B2 (Cat # MAB10833).

Immunofluorescence
Application

Immunofluorescence

Immunofluorescence analysis of HeLa cells using NME1 monoclonal antibody, clone 4B2 (Cat # MAB10833) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

Flow Cytometry
Application

Flow Cytometry

Flow cytometric analysis of Jurkat cells using NME1 monoclonal antibody, clone 4B2 (Cat # MAB10833) (green) and negative control (purple).

  • Specifications

    Product Description

    Mouse monoclonal antibody raised against partial recombinant NME1.

    Immunogen

    Recombinant protein corresponding to human NME1.

    Host

    Mouse

    Reactivity

    Human

    Form

    Liquid

    Isotype

    IgG1

    Recommend Usage

    ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In ascites (0.03% sodium azide)

    Storage Instruction

    Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot (Transfected lysate)

    Western blot analysis using NME1 monoclonal antibody, clone 4B2 (Cat # MAB10833) against NME1-hIgGFc transfected HEK293 cell lysate.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunohistochemical analysis of paraffin-embedded human placenta tissues using NME1 monoclonal antibody, clone 4B2 (Cat # MAB10833).

    Immunofluorescence

    Immunofluorescence analysis of HeLa cells using NME1 monoclonal antibody, clone 4B2 (Cat # MAB10833) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

    Enzyme-linked Immunoabsorbent Assay

    Flow Cytometry

    Flow cytometric analysis of Jurkat cells using NME1 monoclonal antibody, clone 4B2 (Cat # MAB10833) (green) and negative control (purple).
  • Gene Info — NME1

    Entrez GeneID

    4830

    Gene Name

    NME1

    Gene Alias

    AWD, GAAD, NB, NBS, NDPK-A, NDPKA, NM23, NM23-H1

    Gene Description

    non-metastatic cells 1, protein (NM23A) expressed in

    Omim ID

    156490 256700

    Gene Ontology

    Hyperlink

    Gene Summary

    This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq

    Other Designations

    NDP kinase A|OTTHUMP00000174772

  • Interactomes
  • Pathways
  • Diseases
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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