Mouse monoclonal antibody raised against partial recombinant NEDD8.
Recombinant protein corresponding to human NEDD8.
ELISA (1:10000) Western Blot (1:500-1:2000) Immunohistochemistry (1:200-1:1000) Immunofluorescence (1:200-1:1000) Flow cytometry (1:200-1:400) The optimal working dilution should be determined by the end user.
In PBS (0.5% protein stabilizer, 0.05% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using NEDD8 monoclonal antibody, clone 1A7 (Cat # MAB10825) with DAB staining.
Immunofluorescence analysis of HeLa cells using NEDD8 monoclonal antibody, clone 1A7 (Cat # MAB10825) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.