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Last updated: 2016/11/27
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NEDD8 monoclonal antibody, clone 1A7

  • Catalog # : MAB10825
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant NEDD8.
  • Immunogen:
  • Recombinant protein corresponding to human NEDD8.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.5% protein stabilizer, 0.05% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis using NEDD8 monoclonal antibody, clone 1A7 (Cat # MAB10825) against human NEDD8 (aa: 1-81) recombinant protein. (Expected MW is 40 kDa)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using NEDD8 monoclonal antibody, clone 1A7 (Cat # MAB10825) with DAB staining.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of HeLa cells using NEDD8 monoclonal antibody, clone 1A7 (Cat # MAB10825) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA
  • ELISA
  • ELISA measurement of NEDD8 monoclonal antibody, clone 1A7 (Cat # MAB10825).
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HeLa cells using NEDD8 monoclonal antibody, clone 1A7 (Cat # MAB10825) (green) and negative control (red).
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 4738
  • Gene Name:
  • NEDD8
  • Gene Alias:
  • FLJ43224,MGC104393,MGC125896,MGC125897,Nedd-8
  • Gene Description:
  • neural precursor cell expressed, developmentally down-regulated 8
  • Other Designations:
  • -
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