KRT15 monoclonal antibody, clone 6E7
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using KRT15 monoclonal antibody, clone 6E7 (Cat # MAB10723) against A-431 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human tonsil tissues using KRT15 monoclonal antibody, clone 6E7 (Cat # MAB10723) .
Immunofluorescence
Immunofluorescence analysis of HepG2 (upper) and PACN-1 (bottom) cells using KRT15 monoclonal antibody, clone 6E7 (Cat # MAB10723) (green) . Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry
Flow cytometric analysis of PACN-1 cells using KRT15 monoclonal antibody, clone 6E7 (Cat # MAB10723) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant KRT15.
Immunogen
Recombinant protein corresponding to human KRT15.
Host
Mouse
Theoretical MW (kDa)
49
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using KRT15 monoclonal antibody, clone 6E7 (Cat # MAB10723) against A-431 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human tonsil tissues using KRT15 monoclonal antibody, clone 6E7 (Cat # MAB10723) .Immunofluorescence
Immunofluorescence analysis of HepG2 (upper) and PACN-1 (bottom) cells using KRT15 monoclonal antibody, clone 6E7 (Cat # MAB10723) (green) . Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of PACN-1 cells using KRT15 monoclonal antibody, clone 6E7 (Cat # MAB10723) (green) and negative control (purple). -
Gene Info — KRT15
Entrez GeneID
3866Gene Name
KRT15
Gene Alias
CK15, K15, K1CO
Gene Description
keratin 15
Omim ID
148030Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region on chromosome 17q21.2. [provided by RefSeq
Other Designations
OTTHUMP00000165060|cytokeratin 15|keratin-15, basic|keratin-15, beta|type I cytoskeletal 15
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Interactome
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Publication Reference
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Regional Control of Hairless versus Hair-Bearing Skin by Dkk2.
Song Y, Boncompagni AC, Kim SS, Gochnauer HR, Zhang Y, Loots GG, Wu D, Li Y, Xu M, Millar SE.
Cell Reports 2018 Dec; 25(11):2981.
Application：IHC-P, Mouse, Mouse hind paws.
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Regional Control of Hairless versus Hair-Bearing Skin by Dkk2.
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