HK2 monoclonal antibody, clone 3D3
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using HK2 monoclonal antibody, clone 3D3 (Cat # MAB10684) against Jurkat (1) , HeLa (2) and HEK293 (3) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human esophagus cancer tissues (upper) and human lung cancer (bottom) using HK2 monoclonal antibody, clone 3D3 (Cat # MAB10684) with DAB staining.
Flow Cytometry
Flow cytometric analysis of K-562 cells using HK2 monoclonal antibody, clone 3D3 (Cat # MAB10684) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant HK2.
Immunogen
Recombinant protein corresponding to human HK2.
Host
Mouse
Theoretical MW (kDa)
102
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using HK2 monoclonal antibody, clone 3D3 (Cat # MAB10684) against Jurkat (1) , HeLa (2) and HEK293 (3) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human esophagus cancer tissues (upper) and human lung cancer (bottom) using HK2 monoclonal antibody, clone 3D3 (Cat # MAB10684) with DAB staining.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of K-562 cells using HK2 monoclonal antibody, clone 3D3 (Cat # MAB10684) (green) and negative control (purple). -
Gene Info — HK2
Entrez GeneID
3099Gene Name
HK2
Gene Alias
DKFZp686M1669, HKII, HXK2
Gene Description
hexokinase 2
Omim ID
601125Gene Ontology
HyperlinkGene Summary
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq
Other Designations
hexokinase-2, muscle
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Interactome
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Pathway
- Amino sugar and nucleotide sugar metabolism
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Fructose and mannose metabolism
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