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Last updated: 2017/6/25
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HK2 monoclonal antibody, clone 3D3

  • Catalog # : MAB10684
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant HK2.
  • Immunogen:
  • Recombinant protein corresponding to human HK2.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 102
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using HK2 monoclonal antibody, clone 3D3 (Cat # MAB10684) against Jurkat (1) , HeLa (2) and HEK293 (3) cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human esophagus cancer tissues (upper) and human lung cancer (bottom) using HK2 monoclonal antibody, clone 3D3 (Cat # MAB10684) with DAB staining.
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of K-562 cells using HK2 monoclonal antibody, clone 3D3 (Cat # MAB10684) (green) and negative control (purple) .
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 3099
  • Gene Name:
  • HK2
  • Gene Alias:
  • DKFZp686M1669,HKII,HXK2
  • Gene Description:
  • hexokinase 2
  • Gene Summary:
  • Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq
  • Other Designations:
  • hexokinase-2, muscle
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