Immunohistochemical analysis of paraffin-embedded human breast carcinoma (upper) and kidney carcinoma (bottom) , showing cytoplasmic localization using GAPDH monoclonal antibody, clone 1A10 (Cat # MAB10662) with DAB staining.
Confocal immunofluorescence analysis of methanol-fixed HepG2 (upper) and HeLa (bottom) cells using GAPDH monoclonal antibody, clone 1A10 (Cat # MAB10662) (green) , showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.
The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq