DDR2 monoclonal antibody, clone 3B11E4
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot analysis using DDR2 monoclonal antibody, clone 3B11E4 (Cat # MAB10622) against truncated DDR2 recombinant protein.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human skeletal musle tissues using DDR2 monoclonal antibody, clone 3B11E4 (Cat # MAB10622) .
Immunofluorescence
Confocal immunofluorescence analysis of A-549 cells using DDR2 monoclonal antibody, clone 3B11E4 (Cat # MAB10622) (green) . Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant DDR2.
Immunogen
Recombinant protein corresponding to human DDR2.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot analysis using DDR2 monoclonal antibody, clone 3B11E4 (Cat # MAB10622) against truncated DDR2 recombinant protein.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human skeletal musle tissues using DDR2 monoclonal antibody, clone 3B11E4 (Cat # MAB10622) .Immunofluorescence
Confocal immunofluorescence analysis of A-549 cells using DDR2 monoclonal antibody, clone 3B11E4 (Cat # MAB10622) (green) . Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
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Gene Info — DDR2
Entrez GeneID
4921Gene Name
DDR2
Gene Alias
MIG20a, NTRKR3, TKT, TYRO10
Gene Description
discoidin domain receptor tyrosine kinase 2
Omim ID
191311Gene Ontology
HyperlinkGene Summary
Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation, and metabolism. In several cases the biochemical mechanism by which RTKs transduce signals across the membrane has been shown to be ligand induced receptor oligomerization and subsequent intracellular phosphorylation. This autophosphorylation leads to phosphorylation of cytosolic targets as well as association with other molecules, which are involved in pleiotropic effects of signal transduction. RTKs have a tripartite structure with extracellular, transmembrane, and cytoplasmic regions. This gene encodes a member of a novel subclass of RTKs and contains a distinct extracellular region encompassing a factor VIII-like domain. Alternative splicing in the 5' UTR results in multiple transcript variants encoding the same protein. [provided by RefSeq
Other Designations
OTTHUMP00000032332|OTTHUMP00000038368|cell migration-inducing protein 20|discoidin domain receptor family, member 2|hydroxyaryl-protein kinase|migration-inducing gene 16 protein|neurotrophic tyrosine kinase receptor related 3|tyrosylprotein kinase
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Interactome
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Disease
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