Mouse monoclonal antibody raised against partial recombinant AIFM1.
Recombinant protein corresponding to human AIFM1.
Theoretical MW (kDa):
Human, Monkey, Mouse, Rat
ELISA (1:10000) Western Blot (1:500-1:2000) Immunohistochemistry (1:200-1:1000) Immunofluorescence (1:200-1:1000) Flow cytometry (1:200-1:400) The optimal working dilution should be determined by the end user.
In ascites (0.03% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis using AIFM1 monoclonal antibody, clone 4E7 (Cat # MAB10484) against NIH/3T3 (1), Jurkat (2), HeLa (3), HepG2 (4), MOLT 4 (5), C6 (6), Raji (7), COS-7 (8) and PC-12 (9) cell lysate.
Western Blot (Recombinant protein)
Western blot analysis using AIFM1 monoclonal antibody, clone 4E7 (Cat # MAB10484) against recombinant protein.
Immunohistochemical analysis of paraffin-embedded human breast cancer (A), human cervical cancer (B), human rectum cancer (C) and human lung cancer (D) tissues using AIFM1 monoclonal antibody, clone 4E7 (Cat # MAB10484) with DAB staining.
Immunofluorescence analysis of NIH/3T3 cells using AIFM1 monoclonal antibody, clone 4E7 (Cat # MAB10484) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it effects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Several alternative transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq
OTTHUMP00000024007,OTTHUMP00000024008,OTTHUMP00000024009,programmed cell death 8,programmed cell death 8 (apoptosis-inducing factor),striatal apoptosis-inducing factor