Mouse monoclonal antibody raised against recombinant PRKAA1.
Recombinant protein corresponding to human PRKAA1.
Theoretical MW (kDa):
Human, Mouse, Rat
ELISA (1:10000) Western Blot (1:500-1:2000) Immunohistochemistry (1:200-1:1000) Immunofluorescence (1:200-1:1000) Flow cytometry (1:200-1:400) The optimal working dilution should be determined by the end user.
In ascites (0.03% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemical analysis of paraffin-embedded human ovarian cancer (A) and brain tissue (B) using PRKAA1 monoclonal antobody, clone 2B7 (Cat # MAB10302) with DAB staining.
Immunofluorescence analysis of NTERA-2 cells using PRKAA1 monoclonal antobody, clone 2B7 (Cat # MAB10302) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric analysis of PC-2 cells using PRKAA1 monoclonal antobody, clone 2B7 (Cat # MAB10302) (green) and negative control (purple).
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
5'-AMP-activated protein kinase, catalytic alpha-1 chain,AMP -activate kinase alpha 1 subunit,AMP-activated protein kinase, catalytic, alpha-1,AMPK alpha 1