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Last updated: 2017/4/23
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RCHY1 monoclonal antibody, clone 1H10

  • Catalog # : MAB10298
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant RCHY1.
  • Immunogen:
  • Recombinant protein corresponding to human RCHY1.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 30, 60
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using RCHY1 monoclonal antobody, clone 1H10 (Cat # MAB10298) against HeLa (1), A-549 (2), MCF-7 (3) and PC-12 (4) cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using RCHY1 monoclonal antobody, clone 1H10 (Cat # MAB10298) with DAB staining.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of HeLa cells using RCHY1 monoclonal antobody, clone 1H10 (Cat # MAB10298) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of PC-12 cells using RCHY1 monoclonal antobody, clone 1H10 (Cat # MAB10298) (blue) and negative control (red).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • RCHY1
  • Gene Alias:
  • ARNIP,CHIMP,DKFZp586C1620,PIRH2,PRO1996,RNF199,ZNF363,hARNIP
  • Gene Description:
  • ring finger and CHY zinc finger domain containing 1
  • Gene Summary:
  • The protein encoded by this gene has ubiquitin-protein ligase activity. This protein binds with p53 and promotes the ubiquitin-mediated proteosomal degradation of p53. This gene is oncogenic because loss of p53 function contributes directly to malignant tumor development. Transcription of this gene is regulated by p53. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
  • Other Designations:
  • CH-rich interacting match with PLAG1,androgen-receptor N-terminal-interacting protein,zinc finger protein 363
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