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Last updated: 2016/12/4
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LIME1 monoclonal antibody, clone LIME-06

  • Catalog # : MAB0960
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant LIME1.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 141-295 of human LIME1.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 31
  • Reactivity:
  • Human
  • Specificity:
  • This antibody was raised against intracellular fragment corresponding to aa 141-295 of human LIME, a 31 KDa Lck-interacting transmembrane adaptor expressed by T cells.
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. Do not freeze.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Immunoprecipitation
  • Immunoprecipitation
  • Induction of LIME1 tyrosine phosphorylation. Peripheral blood T cells were left unstimulated (-) or stimulated with anti-human CD3 (MEM-92) or anti-human CD4 (MEM-16), and LIME1 was immunoprecipitated from laurylmaltoside lysates with the LIME1 monoclonal antibody, clone LIME-06 (Cat # MAB0960) (immunoaffinity sorbent) and analyzed by Western blotting to visualize tyrosine-phosphorylated LIME1 (top) and total LIME1 (bottom).
  • Application Image
  • Gene Information
  • Gene Name:
  • LIME1
  • Gene Alias:
  • FLJ20406,LIME,LP8067,dJ583P15.4
  • Gene Description:
  • Lck interacting transmembrane adaptor 1
  • Gene Summary:
  • LIME1 is a raft-associated transmembrane adaptor phosphoprotein that is preferentially expressed in hemopoietic cells, particularly T cells (Brdickova et al., 2003 [PubMed 14610046]; Hur et al., 2003 [PubMed 14610044]).[supplied by OMIM
  • Other Designations:
  • OTTHUMP00000031598
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