CK2 Kinase (Human) Assay/Inhibitor Screening Assay Kit is a single-site, non-quantitative immunoassay for CK2 activity. Plates are pre-coated with a substrate corresponding to recombinant p53, which contains a serine residue that are phosphorylated by CK2 (Casein kinase II). The detector antibody specifically detects only the phosphorylated form of serine46 on p53.
Quality Control Testing:
Dose dependent curve
Dose dependent curve of recombinant CK2 enzyme reaction.
Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. While this gene is found on chromosome 20, a related transcribed pseudogene is found on chromosome 11. Three transcript variants encoding two different proteins have been found for this gene. [provided by RefSeq
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