DVL1 & CSNK2A1 Protein Protein Interaction Antibody Pair
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More Files
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Representative image of Proximity Ligation Assay of protein-protein interactions between DVL1 and CSNK2A1. HeLa cells were stained with anti-DVL1 rabbit purified polyclonal antibody 1:1200 and anti-CSNK2A1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
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Specification
Product Description
This protein protein interaction antibody pair set comes with two antibodies to detect the protein-protein interaction, one against the DVL1 protein, and the other against the CSNK2A1 protein for use in in situ Proximity Ligation Assay. See Publication Reference below.
Reactivity
Human
Quality Control Testing
Protein protein interaction immunofluorescence result.
Representative image of Proximity Ligation Assay of protein-protein interactions between DVL1 and CSNK2A1. HeLa cells were stained with anti-DVL1 rabbit purified polyclonal antibody 1:1200 and anti-CSNK2A1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
Supplied Product
Antibody pair set content:
1. DVL1 rabbit purified polyclonal antibody (100 ug)
2. CSNK2A1 mouse monoclonal antibody (40 ug)
*Reagents are sufficient for at least 30-50 assays using recommended protocols.Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
In situ Proximity Ligation Assay (Cell)
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Gene Info — CSNK2A1
Entrez GeneID
1457Gene Name
CSNK2A1
Gene Alias
CK2A1, CKII
Gene Description
casein kinase 2, alpha 1 polypeptide
Omim ID
115440Gene Ontology
HyperlinkGene Summary
Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. While this gene is found on chromosome 20, a related transcribed pseudogene is found on chromosome 11. Three transcript variants encoding two different proteins have been found for this gene. [provided by RefSeq
Other Designations
CK2 catalytic subunit alpha|OTTHUMP00000029925|OTTHUMP00000029926|casein kinase II alpha 1 subunit|casein kinase II alpha subunit|protein kinase CK2
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Gene Info — DVL1
Entrez GeneID
1855Gene Name
DVL1
Gene Alias
DVL, MGC54245
Gene Description
dishevelled, dsh homolog 1 (Drosophila)
Omim ID
601365Gene Ontology
HyperlinkGene Summary
DVL1, the human homolog of the Drosophila dishevelled gene (dsh) encodes a cytoplasmic phosphoprotein that regulates cell proliferation, acting as a transducer molecule for developmental processes, including segmentation and neuroblast specification. DVL1 is a candidate gene for neuroblastomatous transformation. The Schwartz-Jampel syndrome and Charcot-Marie-Tooth disease type 2A have been mapped to the same region as DVL1. The phenotypes of these diseases may be consistent with defects which might be expected from aberrant expression of a DVL gene during development. [provided by RefSeq
Other Designations
OTTHUMP00000003104|dishevelled 1|dishevelled 1 (homologous to Drosophila dsh)
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Interactome
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Pathway
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Disease
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