c-Src Kinase (Human) Assay/Inhibitor Screening Assay Kit
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More Files
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Specification
Product Description
c-Src Kinase (Human) Assay/Inhibitor Screening Assay Kit is a single-site, non-quantitative immunoassay for kinase activity of recombinant catalytic domain of c-Src. Plates are pre-coated with a newly designed "Tyrosine kinase-binding module-1", which can easily bind recombinant catalytic domain of c-Src, subsequently activate c-Src kinase activity on a microtiter plate. The detector antibody is PY-39, an antibody that specifically detects the phosphotyrosine residue on recombinant catalytic domain of c-Src itself, which means that this kit measures the intensity of autophosphorylation of c-Src catalytic domain.
Assay Type
Quantitative
Reactivity
Human
Regulation Status
For research use only (RUO)
Quality Control Testing
Dose dependent curve
Dose dependent curve of recombinant c-Src catalytic domain enzyme reaction.
Storage Instruction
Store the kit at 4°C.
Note
SRC (Human) Recombinant Protein (Cat# P6990) is a Src positive control designed to use for c-Src Kinase (Human) Assay/Inhibitor Screening Assay Kit (Cat# KA0058).
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Applications
Functional Study
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Gene Info — SRC
Entrez GeneID
6714Gene Name
SRC
Gene Alias
ASV, SRC1, c-SRC, p60-Src
Gene Description
v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
Omim ID
190090Gene Ontology
HyperlinkGene Summary
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000174476|OTTHUMP00000174477|proto-oncogene tyrosine-protein kinase SRC|protooncogene SRC, Rous sarcoma|tyrosine kinase pp60c-src|tyrosine-protein kinase SRC-1
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Publication Reference
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Berberine Reduces the Metastasis of Chondrosarcoma by Modulating the αvβ3 Integrin and the PKCδ, c-Src, and AP-1 Signaling Pathways.
Chi-Ming Wu, Te-Mao Li, Tzu-Wei Tan, Yi-Chin Fong, Chih-Hsin Tang.
Evidence-Based Complementary and Alternative Medicine 2013 Aug; 2013:423164.
Application:Func, KA, Human, JJ012, SW1353 cells.
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Lipoteichoic acid enhances IL-6 production in human synovial fibroblasts via TLR2 receptor, PKCdelta and c-Src dependent pathways.
Chih-Hsin Tang, Chin-Jung Hsu, Wei-Hung Yang, Yi-Chin Fong.
Biochemical Pharmacology 2010 Jun; 79(11):1648.
Application:Func, KA, Human, Human synovial fibroblasts.
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Berberine Reduces the Metastasis of Chondrosarcoma by Modulating the αvβ3 Integrin and the PKCδ, c-Src, and AP-1 Signaling Pathways.
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