IBRDC2 monoclonal antibody (M02), clone 4F1
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant IBRDC2.
Immunogen
IBRDC2 (NP_877434, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MGSAGRLHYLAMTAENPTPGDLAPAPLITCKLCLCEQSLDKMTTLQECQCIFCTACLKQYMQLAIREGCGSPITCPDMVCLNHGTLQEAEIACLVPVDQF
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
IBRDC2 monoclonal antibody (M02), clone 4F1 Western Blot analysis of IBRDC2 expression in IMR-32 ( Cat # L008V1 ).Western Blot (Transfected lysate)
Western Blot analysis of RNF144B expression in transfected 293T cell line by IBRDC2 monoclonal antibody (M02), clone 4F1.
Lane 1: RNF144B transfected lysate(33.6 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to IBRDC2 on formalin-fixed paraffin-embedded human cerebellum. [antibody concentration 1.2 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged RNF144B is 3 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to IBRDC2 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — RNF144B
Entrez GeneID
255488GeneBank Accession#
NM_182757Protein Accession#
NP_877434Gene Name
RNF144B
Gene Alias
IBRDC2, KIAA0161, MGC71786, bA528A10.3, p53RFP
Gene Description
ring finger protein 144B
Gene Ontology
HyperlinkOther Designations
IBR domain containing 2|OTTHUMP00000016079|OTTHUMP00000016080|OTTHUMP00000039360|p53-inducible RING finger protein|ring finger 144B
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Disease
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