SFRS12 monoclonal antibody (M01), clone 1D11
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant SFRS12.
Immunogen
SFRS12 (NP_631907, 443 a.a. ~ 508 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
NDRDGKEKLEKNSTSLKEKEHNKEPDSSVSKEVDDKDAPRTEENKIQHNGNCQLNEENLSTKTEAV
Host
Mouse
Reactivity
Human
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (33 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of SFRS12 expression in transfected 293T cell line by SFRS12 monoclonal antibody (M01), clone 1D11.
Lane 1: SFRS12 transfected lysate (Predicted MW: 59.4 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SFRS12 is 0.3 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to SFRS12 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — SFRS12
Entrez GeneID
140890GeneBank Accession#
NM_139168Protein Accession#
NP_631907Gene Name
SFRS12
Gene Alias
DKFZp564B176, MGC133045, SRrp508, SRrp86
Gene Description
splicing factor, arginine/serine-rich 12
Omim ID
609268Gene Ontology
HyperlinkGene Summary
SFRS12 belongs to the superfamily of serine/arginine-rich (SR) splicing factors. It modulates splice site selection by regulating the activities of other SR proteins (Barnard et al., 2002 [PubMed 11991645]).[supplied by OMIM
Other Designations
OTTHUMP00000123466|serine-arginine-rich splicing regulatory protein 508
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