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CDCA1 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00083540-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-CDCA1 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 51.15
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-CDCA1 antibody (H00083540-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00083540-T01
    NUF2 transfected lysate.
    Western Blot
    QC Testing of H00083540-T01
    Lane 1: NUF2 transfected lysate ( 54.3 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Gene Name:
  • NUF2
  • Gene Alias:
  • CDCA1,NUF2R
  • Gene Description:
  • NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae)
  • Gene Summary:
  • This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq
  • Other Designations:
  • NUF2, NDC80 kinetochore complex component,OTTHUMP00000032177,OTTHUMP00000032178,cell division cycle associated 1
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