CDCA1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
NUF2 transfected lysate.
Western Blot
Lane 1: NUF2 transfected lysate ( 54.3 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CDCA1 full-length
Host
Human
Theoretical MW (kDa)
51.15
Interspecies Antigen Sequence
Mouse (73); Rat (79)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CDCA1 antibody (H00083540-B01) by Western Blots.
SDS-PAGE Gel
NUF2 transfected lysate.
Western Blot
Lane 1: NUF2 transfected lysate ( 54.3 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NUF2
Entrez GeneID
83540GeneBank Accession#
BC008489Protein Accession#
AAH08489Gene Name
NUF2
Gene Alias
CDCA1, NUF2R
Gene Description
NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae)
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq
Other Designations
NUF2, NDC80 kinetochore complex component|OTTHUMP00000032177|OTTHUMP00000032178|cell division cycle associated 1
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Interactome
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Disease
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