ALS2CR8 monoclonal antibody (M01), clone 2A3
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Specifications
Product Description
Mouse monoclonal antibody raised against a partial recombinant ALS2CR8.
Immunogen
ALS2CR8 (NP_079020, 621 a.a. ~ 718 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
CLTQNNSTASTMGNLPEPDQNLVAMDELVEVGDVEDTGNLEGTVHRILLGDVQTIPIQIIDNHSALIEENPESTISVSQVKQEPKEPALSMEAKNCGL
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (71); Rat (79)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.52 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
ALS2CR8 monoclonal antibody (M01), clone 2A3 Western Blot analysis of ALS2CR8 expression in Hela S3 NE ( Cat # L013V3 ).Western Blot (Transfected lysate)
Western Blot analysis of ALS2CR8 expression in transfected 293T cell line by ALS2CR8 monoclonal antibody (M01), clone 2A3.
Lane 1: ALS2CR8 transfected lysate(79.8 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to ALS2CR8 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 1 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ALS2CR8 is approximately 0.3ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of ALS2CR8 over-expressed 293 cell line, cotransfected with ALS2CR8 Validated Chimera RNAi ( Cat # H00079800-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ALS2CR8 monoclonal antibody (M01), clone 2A3 (Cat # H00079800-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Immunofluorescence
Immunofluorescence of monoclonal antibody to ALS2CR8 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — ALS2CR8
Entrez GeneID
79800GeneBank Accession#
NM_024744Protein Accession#
NP_079020Gene Name
ALS2CR8
Gene Alias
CARF, DKFZp667N246, FLJ12675, FLJ21579, NYD-SP24
Gene Description
amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 8
Omim ID
607586Gene Ontology
HyperlinkGene Summary
O
Other Designations
calcium-response factor
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