ALG12 monoclonal antibody (M06), clone 5E3
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant ALG12.
Immunogen
ALG12 (NP_077010, 369 a.a. ~ 425 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
NYPGGVAMQRLHQLVPPQTDVLLHIDVAAAQTGVSRFLQVNSAWRYDKREDVQPGTG
Host
Mouse
Reactivity
Human, Rat
Interspecies Antigen Sequence
Mouse (78); Rat (78)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (32.01 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
ALG12 monoclonal antibody (M06), clone 5E3. Western Blot analysis of ALG12 expression in PC-12 ( Cat # L012V1 ).Western Blot (Cell lysate)
ALG12 monoclonal antibody (M06), clone 5E3 Western Blot analysis of ALG12 expression in HeLa ( Cat # L013V1 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ALG12 is approximately 0.3ng/ml as a capture antibody.ELISA
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Gene Info — ALG12
Entrez GeneID
79087GeneBank Accession#
NM_024105Protein Accession#
NP_077010Gene Name
ALG12
Gene Alias
ECM39, MGC111358, MGC3136, PP14673, hALG12
Gene Description
asparagine-linked glycosylation 12, alpha-1,6-mannosyltransferase homolog (S. cerevisiae)
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the glycosyltransferase 22 family. The encoded protein catalyzes the addition of the eighth mannose residue in an alpha-1,6 linkage onto the dolichol-PP-oligosaccharide precursor (dolichol-PP-Man(7)GlcNAc(2)) required for protein glycosylation. Mutations in this gene have been associated with congenital disorder of glycosylation type Ig (CDG-Ig)characterized by abnormal N-glycosylation. [provided by RefSeq
Other Designations
alpha-1,6-mannosyltransferase ALG12|asparagine-linked glycosylation 12 homolog (S. cerevisiae, alpha-1,6-mannosyltransferase)|asparagine-linked glycosylation 12 homolog (yeast, alpha-1,6-mannosyltransferase)|dolichyl-P-mannose:Man-7-GlcNAc-2-PP-dolichyl-a
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Interactome
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Pathway
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