CENPM monoclonal antibody (M01), clone 4C12-2C8
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More Files
- More Functions
Western Blot (Cell lysate)
C22orf18 monoclonal antibody (M01), clone 4C12-2C8 Western Blot analysis of C22orf18 expression in K-562 ( Cat # L009V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of CENPM expression in transfected 293T cell line by C22orf18 monoclonal antibody (M01), clone 4C12-2C8.
Lane 1: CENPM transfected lysate (Predicted MW: 19.7 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of CENPM transfected lysate using anti-CENPM monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CENPM MaxPab rabbit polyclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged C22orf18 is approximately 0.03ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to MGC861 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (45.54 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant CENPM.
Immunogen
CENPM (AAH00705, 1 a.a. ~ 180 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MSVLRPLDKLPGLNTATILLVGTEDALLQQLADSMLKEDCASELKVHLAKSLPLPSSVNRPRIDLIVFVVNLHSKYSLQNTEESLRHVDASFFLGKVCFLATGAGRESHCSIHRHTVVKLAHTYQSPLLYCDLEVEGFRATMAQRLVRVLQICAGHVPGVSALNLLSLLRSSEGPSLEDL
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (79); Rat (80)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (45.54 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
C22orf18 monoclonal antibody (M01), clone 4C12-2C8 Western Blot analysis of C22orf18 expression in K-562 ( Cat # L009V1 ).Western Blot (Transfected lysate)
Western Blot analysis of CENPM expression in transfected 293T cell line by C22orf18 monoclonal antibody (M01), clone 4C12-2C8.
Lane 1: CENPM transfected lysate (Predicted MW: 19.7 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of CENPM transfected lysate using anti-CENPM monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CENPM MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged C22orf18 is approximately 0.03ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to MGC861 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — CENPM
Entrez GeneID
79019GeneBank Accession#
BC000705Protein Accession#
AAH00705Gene Name
CENPM
Gene Alias
C22orf18, CENP-M, MGC861, PANE1, bK250D10.2
Gene Description
centromere protein M
Omim ID
610152Gene Ontology
HyperlinkGene Summary
The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). CENPM is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).[supplied by OMIM
Other Designations
OTTHUMP00000028741|proliferation associated nuclear element 1
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Interactome
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Disease
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