LEPRE1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
LEPRE1 transfected lysate
Western Blot
Lane 1: LEPRE1 transfected lysate ( 42.9 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-LEPRE1 full-length
Host
Human
Theoretical MW (kDa)
42.9
Interspecies Antigen Sequence
Mouse (91); Rat (92)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-LEPRE1 antibody (H00064175-B01) by Western Blots.
SDS-PAGE Gel
LEPRE1 transfected lysate
Western Blot
Lane 1: LEPRE1 transfected lysate ( 42.9 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — LEPRE1
Entrez GeneID
64175GeneBank Accession#
BC015309Protein Accession#
AAH15309Gene Name
LEPRE1
Gene Alias
GROS1, MGC117314, P3H1
Gene Description
leucine proline-enriched proteoglycan (leprecan) 1
Gene Ontology
HyperlinkGene Summary
This gene encodes an enzyme that is a member of the collagen prolyl hydroxylase family. These enzymes are localized to the endoplasmic reticulum and their activity is required for proper collagen synthesis and assembly. Mutations in this gene are associated with osteogenesis imperfecta type VIII. Two alternatively spliced transcript variants encoding different isoforms have been described. Other variants may exist, but their biological validity has not been determined. [provided by RefSeq
Other Designations
OTTHUMP00000008736|growth suppressor 1|leprecan|leprecan 1|prolyl 3-hydroxylase 1
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