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Last updated: 2016/11/27

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MCM10 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00055388-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-MCM10 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 96.25
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-MCM10 antibody (H00055388-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00055388-T01
    MCM10 transfected lysate.
    Western Blot
    QC Testing of H00055388-T01
    Lane 1: MCM10 transfected lysate ( 96.25 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Gene Name:
  • MCM10
  • Gene Alias:
  • CNA43,DNA43,MGC126776,PRO2249
  • Gene Description:
  • minichromosome maintenance complex component 10
  • Gene Summary:
  • The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq
  • Other Designations:
  • MCM10 minichromosome maintenance deficient 10,OTTHUMP00000019133,homolog of yeast MCM10
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