Proximity Ligation Analysis of protein-protein interactions between SUFU and GLI1. HeLa cells were stained with anti-SUFU rabbit purified polyclonal 1:1200 and anti-GLI1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
SUFU encodes a component of the Sonic hedgehog (SHH; MIM 600725)/Patched (PTCH; MIM 601309) signaling pathway. Mutations in genes encoding components of this pathway are deleterious for normal development and are associated with cancer-predisposing syndromes (e.g., HPE3, MIM 142945; BCNS, MIM 109400; and GCPS, MIM 175700).[supplied by OMIM
OTTHUMP00000020374,OTTHUMP00000059161,suppressor of fused