WBP2 monoclonal antibody (M02), clone 3B1
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of WBP2 expression in transfected 293T cell line by WBP2 monoclonal antibody (M02), clone 3B1.
Lane 1: WBP2 transfected lysate (Predicted MW: 28.1 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of monoclonal antibody to WBP2 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (54.45 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant WBP2.
Immunogen
WBP2 (AAH10616, 1 a.a. ~ 261 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MALNKNHSEGGGVIVNNTESILMSYDHVELTFNDMKNVPEAFKGTKKGTVYLTPYRVIFLSKGKDAMQSFMMPFYLMKDCEIKQPVFGANYIKGTVKAEAGGGWEGSASYKLTFTAGGAIEFGQRMLQVASQASRGEVPSGAYGYSYMPSGAYVYPPPVANGMYPCPPGYPYPPPPPEFYPGPPMMDGAMGYVQPPPPPYPGPMEPPVSGPDVPSTPAAEAKAAEAAASAYYNPGNPHNVYMPTSQPPPPPYYPPEDKKTQ
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (97); Rat (96)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (54.45 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of WBP2 expression in transfected 293T cell line by WBP2 monoclonal antibody (M02), clone 3B1.
Lane 1: WBP2 transfected lysate (Predicted MW: 28.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to WBP2 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — WBP2
Entrez GeneID
23558GeneBank Accession#
BC010616Protein Accession#
AAH10616Gene Name
WBP2
Gene Alias
MGC18269, WBP-2
Gene Description
WW domain binding protein 2
Omim ID
606962Gene Ontology
HyperlinkGene Summary
The globular WW domain is composed of 38 to 40 semiconserved amino acids shared by proteins of diverse functions including structural, regulatory, and signaling proteins. The domain is involved in mediating protein-protein interactions through the binding of polyproline ligands. This gene encodes a WW domain binding protein, which binds to the WW domain of Yes kinase-associated protein by its PY motifs. The function of this protein has not been determined. [provided by RefSeq
Other Designations
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Interactome
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Publication Reference
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Tyrosine phosphorylation of transcriptional coactivator WW-domain binding protein 2 regulates estrogen receptor α function in breast cancer via the Wnt pathway.
Lim SK, Orhant-Prioux M, Toy W, Tan KY, Lim YP.
FASEB Journal 2011 Sep; 25(9):3004.
Application：IP, Human, MCF-7 cells.
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WW domain-mediated interaction with Wbp2 is important for the oncogenic property of TAZ.
Chan SW, Lim CJ, Huang C, Chong YF, Gunaratne HJ, Hogue KA, Blackstock WP, Harvey KF, Hong W.
Oncogene 2011 Feb; 30(5):600.
Application：WB-Ce, Human, MCF 10A cells.
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Tyrosine phosphorylation of transcriptional coactivator WW-domain binding protein 2 regulates estrogen receptor α function in breast cancer via the Wnt pathway.
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