NUDCD3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
NUDCD3 transfected lysate.
Western Blot
Lane 1: NUDCD3 transfected lysate ( 24.7 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NUDCD3 full-length
Host
Human
Theoretical MW (kDa)
24.7
Interspecies Antigen Sequence
Mouse (90)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NUDCD3 antibody (H00023386-B01P) by Western Blots.
SDS-PAGE Gel
NUDCD3 transfected lysate.
Western Blot
Lane 1: NUDCD3 transfected lysate ( 24.7 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NUDCD3
Entrez GeneID
23386GeneBank Accession#
BC003691Protein Accession#
AAH03691.1Gene Name
NUDCD3
Gene Alias
KIAA1068, NudCL
Gene Description
NudC domain containing 3
Omim ID
610296Gene Ontology
HyperlinkGene Summary
The product of this gene functions to maintain the stability of dynein intermediate chain. Depletion of this gene product results in aggregation and degradation of dynein intermediate chain, mislocalization of the dynein complex from kinetochores, spindle microtubules, and spindle poles, and loss of gamma-tubulin from spindle poles. The protein localizes to the Golgi apparatus during interphase, and levels of the protein increase after the G1/S transition. [provided by RefSeq
Other Designations
NudC-like protein|OTTHUMP00000159457
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Interactome
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