STON1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
STON1 transfected lysate.
Western Blot
Lane 1: STON1 transfected lysate ( 80.96 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-STON1 full-length
Host
Human
Theoretical MW (kDa)
80.96
Interspecies Antigen Sequence
Mouse (76); Rat (76)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-STON1 antibody (H00011037-B01) by Western Blots.
SDS-PAGE Gel
STON1 transfected lysate.
Western Blot
Lane 1: STON1 transfected lysate ( 80.96 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — STON1
Entrez GeneID
11037GeneBank Accession#
NM_006873.2Protein Accession#
NP_006864.2Gene Name
STON1
Gene Alias
DKFZp781K2462, MGC149803, MGC149804, SBLF, STN1, STNB1, stoned-b1
Gene Description
stonin 1
Omim ID
605357Gene Ontology
HyperlinkGene Summary
Endocytosis of cell surface proteins is mediated by a complex molecular machinery that assembles on the inner surface of the plasma membrane. This gene encodes one of two human homologs of the Drosophila melanogaster stoned B protein. This protein is related to components of the endocytic machinery and exhibits a modular structure consisting of an N-terminal proline-rich domain, a central region of homology specific to the human stoned B-like proteins, and a C-terminal region homologous to the mu subunits of adaptor protein (AP) complexes. Co-transcription of this gene and the neighboring downstream gene generates a rare transcript (SALF), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq
Other Designations
stoned B homolog 1|stoned B-like factor
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Interactome
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Pathway
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