SC65 monoclonal antibody (M01), clone 1E12
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of SC65 expression in transfected 293T cell line by SC65 monoclonal antibody (M01), clone 1E12.
Lane 1: SC65 transfected lysate (Predicted MW: 50.381 KDa).
Lane 2: Non-transfected lysate.
Western Blot detection against Immunogen (37.84 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant SC65.
Immunogen
SC65 (NP_006446, 270 a.a. ~ 379 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
QCKVDCEANLTPNVGGYFVDKFVATMYHYLQFAYYKLNDVRQAARSAASYMLFDPKDSVMQQNLVYYRFHRARWGLEEEDFQPREEAMLYHNQTAELRELLEFTHMYLQS
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (91)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.84 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of SC65 expression in transfected 293T cell line by SC65 monoclonal antibody (M01), clone 1E12.
Lane 1: SC65 transfected lysate (Predicted MW: 50.381 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
ELISA
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Gene Info — SC65
Entrez GeneID
10609GeneBank Accession#
NM_006455Protein Accession#
NP_006446Gene Name
SC65
Gene Alias
NOL55
Gene Description
synaptonemal complex protein SC65
Gene Ontology
HyperlinkGene Summary
This nucleolar protein was first characterized because it was an autoantigen in cases on interstitial cystitis. The protein, with a predicted molecular weight of 50 kDa, appears to be localized in the particulate compartment of the interphase nucleolus, with a distribution distinct from that of nucleolar protein B23. During mitosis it is associated with chromosomes. [provided by RefSeq
Other Designations
OTTHUMP00000164758|nucleolar autoantigen No55|nucleolar autoantigen, 55kDa
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