PCDHGA8 monoclonal antibody (M01), clone 1C11
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More Files
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Specifications
Product Description
Mouse monoclonal antibody raised against a partial recombinant PCDHGA8.
Immunogen
PCDHGA8 (NP_114477, 357 a.a. ~ 444 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
LENSLPGTVIAFLSVHDQDSGKNGQVVCYTRDNLPFKLEKSIGNYYRLVTRKYLDRENVSIYNITVMASDLGTPPLSTETQIALHVAD
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (81); Rat (81)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (35.42 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of PCDHGA8 expression in transfected 293T cell line by PCDHGA8 monoclonal antibody (M01), clone 1C11.
Lane 1: PCDHGA8 transfected lysate(101.48 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PCDHGA8 is approximately 0.3ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of PCDHGA8 over-expressed 293 cell line, cotransfected with PCDHGA8 Validated Chimera RNAi ( Cat # H00009708-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PCDHGA8 monoclonal antibody (M01), clone 1C11 (Cat # H00009708-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — PCDHGA8
Entrez GeneID
9708GeneBank Accession#
NM_032088Protein Accession#
NP_114477Gene Name
PCDHGA8
Gene Alias
KIAA0327, PCDH-GAMMA-A8
Gene Description
protocadherin gamma subfamily A, 8
Omim ID
606295Gene Ontology
HyperlinkGene Summary
This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes. [provided by RefSeq
Other Designations
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