BAG5 monoclonal antibody (M01), clone 2E2
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More Files
- More Functions
Western Blot (Cell lysate)
BAG5 monoclonal antibody (M01), clone 2E2. Western Blot analysis of BAG5 expression in HeLa.
Western Blot (Transfected lysate)
Western Blot analysis of BAG5 expression in transfected 293T cell line by BAG5 monoclonal antibody (M01), clone 2E2.
Lane 1: BAG5 transfected lysate (Predicted MW: 51.2 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged BAG5 is 0.3 ng/ml as a capture antibody.
Western Blot detection against Immunogen (74.91 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant BAG5.
Immunogen
BAG5 (AAH50551, 1 a.a. ~ 447 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MDMGNQHPSISRLQEIQKEVKSVEQQVIGFSGLSDDKNYKKLERILTKQLFEIDSVDTEGKGDIQQARKRAAQETERLLKELEQNANHPHRIEIQNIFEEAQSLVREKIVPFYNGGNCVTDEFEEGIQDIILRLTHVKTGGKISLRKARYHTLTKIWAVQEIIEDCMKKQPSLPLSEDAHPSVAKINFVMCEVNKARGVLIALLMGVNNNETCRHLSCVLSGLIADLDALDVCGRTEIRNYRREVVEDINKLLKYLDLEEEADTTKAFDLRQNHSILKIEKVLKRMREIKNELLQAQNPSELYLSSKTELQGLIGQLDEVSLEKNPCIREARRRAVIEVQTLITYIDLKEALEKRKLFACEEHPSHKAVWNVLGNLSEIQGEVLSFDGNRTDKNYIRLEELLTKQLLALDAVDPQGEEKCKAARKQAVRLAQNILSYLDLKSDEWEY
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (91); Rat (90)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (74.91 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
BAG5 monoclonal antibody (M01), clone 2E2. Western Blot analysis of BAG5 expression in HeLa.Western Blot (Transfected lysate)
Western Blot analysis of BAG5 expression in transfected 293T cell line by BAG5 monoclonal antibody (M01), clone 2E2.
Lane 1: BAG5 transfected lysate (Predicted MW: 51.2 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged BAG5 is 0.3 ng/ml as a capture antibody.ELISA
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Gene Info — BAG5
Entrez GeneID
9529GeneBank Accession#
BC050551Protein Accession#
AAH50551Gene Name
BAG5
Gene Alias
BAG-5
Gene Description
BCL2-associated athanogene 5
Omim ID
603885Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the BAG1-related protein family. BAG1 is an anti-apoptotic protein that functions through interactions with a variety of cell apoptosis and growth related proteins including BCL-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors and members of the heat shock protein 70 kDa family. This protein contains a BAG domain near the C-terminus, which could bind and inhibit the chaperone activity of Hsc70/Hsp70. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq
Other Designations
BAG-family molecular chaperone regulator-5
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Interactome
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Disease
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