BAG5 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
BAG5 polyclonal antibody (Cat # PAB6189)(2 ug/mL) staining of nuclear HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of BAG5.
Immunogen
A synthetic peptide corresponding to N-terminus of human BAG5.
Sequence
DMGNQHPSISRLQ-C
Host
Goat
Theoretical MW (kDa)
56, 51.2
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:64000)
Western blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
BAG5 polyclonal antibody (Cat # PAB6189)(2 ug/mL) staining of nuclear HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — BAG5
Entrez GeneID
9529Protein Accession#
NP_001015049.1;NP_004864.1;NP_001015048.1Gene Name
BAG5
Gene Alias
BAG-5
Gene Description
BCL2-associated athanogene 5
Omim ID
603885Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the BAG1-related protein family. BAG1 is an anti-apoptotic protein that functions through interactions with a variety of cell apoptosis and growth related proteins including BCL-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors and members of the heat shock protein 70 kDa family. This protein contains a BAG domain near the C-terminus, which could bind and inhibit the chaperone activity of Hsc70/Hsp70. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq
Other Designations
BAG-family molecular chaperone regulator-5
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Interactome
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Disease
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Publication Reference
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An evolutionarily conserved family of Hsp70/Hsc70 molecular chaperone regulators.
Takayama S, Xie Z, Reed JC.
The Journal of Biological Chemistry 1999 Jan; 274(2):781.
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An evolutionarily conserved family of Hsp70/Hsc70 molecular chaperone regulators.
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