PCAF monoclonal antibody (M03), clone 5E10
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant PCAF.
Immunogen
PCAF (AAH60823, 353 a.a. ~ 452 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
LEEEVYSQNSPIWDQDFLSASSRTSQLGIQTVINPPPVAGTISYNSTSSSLEQPNAGSSSPACKASSGLEANPGEKRKMTDSHVLEEAKKPRVMGDIPME
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.63 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
PCAF monoclonal antibody (M03), clone 5E10 Western Blot analysis of PCAF expression in Hela S3 NE ( Cat # L013V3 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PCAF is approximately 10ng/ml as a capture antibody.ELISA
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Gene Info — KAT2B
Entrez GeneID
8850GeneBank Accession#
BC060823Protein Accession#
AAH60823Gene Name
KAT2B
Gene Alias
CAF, P, P/CAF, PCAF
Gene Description
K(lysine) acetyltransferase 2B
Omim ID
602303Gene Ontology
HyperlinkGene Summary
CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation. [provided by RefSeq
Other Designations
CREBBP-associated factor|p300/CBP-associated factor
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Interactome
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Disease
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