CRADD monoclonal antibody (M01), clone 1F8
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More Files
- More Functions
Western Blot (Cell lysate)
CRADD monoclonal antibody (M01), clone 1F8 Western Blot analysis of CRADD expression in K-562 ( Cat # L009V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of CRADD expression in transfected 293T cell line by CRADD monoclonal antibody (M01), clone 1F8.
Lane 1: CRADD transfected lysate(23 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CRADD is approximately 0.1ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to CRADD on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (47.63 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant CRADD.
Immunogen
CRADD (AAH37905, 1 a.a. ~ 199 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MEARDKQVLRSLRLELGAEVLVEGLVLQYVYEEGILTENHIQEINAQTTGLRKTMLLLDILPSRGPKAFDTFLDSLQEFPWVREKLKKAREEAMTDLPAGDRLTGIPSHILNSSPSDRQINQLAQRLGPEWEPMVLSLGLSQTDIYRCKANHPHNVQSQVVEAFIRWRQRFGKQATFQSLHNGLRAVEVDPSLLLHMLE
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (89)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (47.63 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
CRADD monoclonal antibody (M01), clone 1F8 Western Blot analysis of CRADD expression in K-562 ( Cat # L009V1 ).Western Blot (Transfected lysate)
Western Blot analysis of CRADD expression in transfected 293T cell line by CRADD monoclonal antibody (M01), clone 1F8.
Lane 1: CRADD transfected lysate(23 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CRADD is approximately 0.1ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to CRADD on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — CRADD
Entrez GeneID
8738GeneBank Accession#
BC037905Protein Accession#
AAH37905Gene Name
CRADD
Gene Alias
MGC9163, RAIDD
Gene Description
CASP2 and RIPK1 domain containing adaptor with death domain
Omim ID
603454Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a death domain (CARD/DD)-containing protein and has been shown to induce cell apoptosis. Through its CARD domain, this protein interacts with, and thus recruits, caspase 2/ICH1 to the cell death signal transduction complex that includes tumor necrosis factor receptor 1 (TNFR1A), RIPK1/RIP kinase, and numbers of other CARD domain-containing proteins. [provided by RefSeq
Other Designations
RIP-associated ICH1/CED3-homologous protein with death domain|RIP-associated protein with a death domain|caspase and RIP adaptor with death domain|death adaptor molecule RAIDD|death domain containing protein CRADD
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Interactome
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Disease
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Publication Reference
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Mutations in CRADD Result in Reduced Caspase-2-Mediated Neuronal Apoptosis and Cause Megalencephaly with a Rare Lissencephaly Variant.
Di Donato N, Jean YY, Maga AM, Krewson BD, Shupp AB, Avrutsky MI, Roy A, Collins S, Olds C, Willert RA, Czaja AM, Johnson R, Stover JA, Gottlieb S, Bartholdi D, Rauch A, Goldstein A, Boyd-Kyle V, Aldinger KA, Mirzaa GM, Nissen A, Brigatti KW, Puffenberger EG, Millen KJ, Strauss KA, Dobyns WB, Troy CM, Jinks RN.
American Journal of Human Genetics 2016 Oct; 99(5):1117.
Application：WB, Human Rat, Human dermal fibroblast, Rat PC12 cells.
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Mutations in CRADD Result in Reduced Caspase-2-Mediated Neuronal Apoptosis and Cause Megalencephaly with a Rare Lissencephaly Variant.
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