EED 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
EED transfected lysate.
Western Blot
Lane 1: EED transfected lysate ( 50.2 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-EED full-length
Host
Human
Theoretical MW (kDa)
50.2
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-EED antibody (H00008726-B01) by Western Blots.
SDS-PAGE Gel
EED transfected lysate.
Western Blot
Lane 1: EED transfected lysate ( 50.2 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — EED
Entrez GeneID
8726GeneBank Accession#
NM_003797.2Protein Accession#
-Gene Name
EED
Gene Alias
HEED, WAIT1
Gene Description
embryonic ectoderm development
Omim ID
605984Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq
Other Designations
WD protein associating with integrin cytoplasmic tails 1
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