NUMB monoclonal antibody (M01), clone 4A7-A6
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of NUMB expression in transfected 293T cell line by NUMB monoclonal antibody (M01), clone 4A7-A6.
Lane 1: NUMB transfected lysate(14.5 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged NUMB is 0.1 ng/ml as a capture antibody.
Western Blot detection against Immunogen (40.59 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant NUMB.
Immunogen
NUMB (AAH33824, 1 a.a. ~ 135 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MPYPAPNVPVVGITPSQMVANVFGTAGHPQAAHPHQSPSLVRQQTFPHYEASSATTSPFFKPPAQHLNGSAAFNGVDDGRLASADRHTEVPTGTCPVDPFEAQWAALENKSKQRTNPSPTNPFSSDLQKTFEIEL
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (90); Rat (84)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (40.59 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of NUMB expression in transfected 293T cell line by NUMB monoclonal antibody (M01), clone 4A7-A6.
Lane 1: NUMB transfected lysate(14.5 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged NUMB is 0.1 ng/ml as a capture antibody.ELISA
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Gene Info — NUMB
Entrez GeneID
8650GeneBank Accession#
BC033824Protein Accession#
AAH33824Gene Name
NUMB
Gene Alias
S171
Gene Description
numb homolog (Drosophila)
Omim ID
603728Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
numb homolog
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Interactome
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Pathway
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Disease
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