TRIM25 (Human) IP-WB Antibody Pair
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More Files
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Immunoprecipitation of TRIM25 transfected lysate using rabbit polyclonal anti-TRIM25 and Protein A Magnetic Bead (U0007), and immunoblotted with mouse purified polyclonal anti-TRIM25.
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Specification
Product Description
This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.
Reactivity
Human
Interspecies Antigen Sequence
Mouse (73); Rat (71)
Quality Control Testing
Immunoprecipitation-Western Blot (IP-WB)
Immunoprecipitation of TRIM25 transfected lysate using rabbit polyclonal anti-TRIM25 and Protein A Magnetic Bead (U0007), and immunoblotted with mouse purified polyclonal anti-TRIM25.
Supplied Product
Antibody pair set content:
1. Antibody pair for IP: rabbit polyclonal anti-TRIM25 (300 ul)
2. Antibody pair for WB: mouse purified polyclonal anti-TRIM25 (50 ug)
Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
Immunoprecipitation-Western Blot
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Gene Info — TRIM25
Entrez GeneID
7706Gene Name
TRIM25
Gene Alias
EFP, RNF147, Z147, ZNF147
Gene Description
tripartite motif-containing 25
Omim ID
600453Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. [provided by RefSeq
Other Designations
estrogen-responsive finger protein|tripartite motif protein TRIM25|zinc finger protein 147 (estrogen-responsive finger protein)|zinc finger protein-147
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Interactome
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Disease
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