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Last updated: 2017/2/26
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VIM monoclonal antibody (M01), clone 3E9

  • Catalog # : H00007431-M01
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against a partial recombinant VIM.
  • Immunogen:
  • VIM (AAH30573, 41 a.a. ~ 140 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
  • Sequence:
  • GSALRPSTSRSLYASSPGGVYATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKG
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Isotype:
  • IgG2a Kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.

    QC Testing of H00007431-M01
    Western Blot detection against Immunogen (36.74 KDa) .
  • Storage Buffer:
  • In 1x PBS, pH 7.4
  • Storage Instruction:
  • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • VIM monoclonal antibody (M01), clone 3E9 Western Blot analysis of VIM expression in HeLa ( Cat # L013V1 ).
  • PDF DownloadProtocol Download
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot analysis of VIM expression in transfected 293T cell line by VIM monoclonal antibody (M01), clone 3E9.

    Lane 1: VIM transfected lysate(53.7 KDa).
    Lane 2: Non-transfected lysate.
  • PDF DownloadProtocol Download
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence of monoclonal antibody to VIM on U-2 OS cell. [antibody concentration 10 ug/ml]
  • PDF DownloadProtocol Download
  • Immunoprecipitation
  • Immunoprecipitation
  • Immunoprecipitation of VIM transfected lysate using anti-VIM monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with VIM MaxPab rabbit polyclonal antibody.
  • PDF DownloadProtocol Download
  • Sandwich ELISA (Recombinant protein)
  • Detection limit for recombinant GST tagged VIM is approximately 10ng/ml as a capture antibody.
  • PDF DownloadProtocol Download
  • ELISA
  • Immunofluorescence (Circulating Tumor Cell)
  • Immunofluorescence (Circulating Tumor Cell)
  • A-549 cells were stained with VIM-FITC labeled monoclonal antibody (Green). The cell nucleus were counterstained with DAPI (Blue).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • ELISA
  • Immunofluorescence (Circulating Tumor Cell)
  • Immunofluorescence (Circulating Tumor Cell)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 7431
  • Gene Name:
  • VIM
  • Gene Alias:
  • FLJ36605
  • Gene Description:
  • vimentin
  • Gene Summary:
  • This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract
  • Other Designations:
  • OTTHUMP00000019224
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