Western blot analysis of TCF12 over-expressed 293 cell line, cotransfected with TCF12 Validated Chimera RNAi ( Cat # H00006938-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TCF12 monoclonal antibody (M01), clone 2E9 (Cat # H00006938-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq