SPI1 monoclonal antibody (M01), clone 1A3
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of SPI1 expression in transfected 293T cell line by SPI1 monoclonal antibody (M01), clone 1A3.
Lane 1: SPI1 transfected lysate(31.083 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SPI1 is approximately 10ng/ml as a capture antibody.
Western Blot detection against Immunogen (38.94 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant SPI1.
Immunogen
SPI1 (NP_003111, 1 a.a. ~ 120 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MEGFPLVPPPSEDLVPYDTDLYQRQTHEYYPYLSSDGESHSDHYWDFHPHHVHSEFESFAENNFTELQSVQPPQLQQLYRHMELEQMHVLDTPMVPPHPSLGHQVSYLPRMCLQYPSLSP
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (38.94 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of SPI1 expression in transfected 293T cell line by SPI1 monoclonal antibody (M01), clone 1A3.
Lane 1: SPI1 transfected lysate(31.083 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SPI1 is approximately 10ng/ml as a capture antibody.ELISA
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Gene Info — SPI1
Entrez GeneID
6688GeneBank Accession#
NM_003120Protein Accession#
NP_003111Gene Name
SPI1
Gene Alias
OF, PU.1, SFPI1, SPI-1, SPI-A
Gene Description
spleen focus forming virus (SFFV) proviral integration oncogene spi1
Omim ID
165170Gene Ontology
HyperlinkGene Summary
This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
31 kDa transforming protein|SPI-1 proto-oncogene|hematopoietic transcription factor PU.1
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Interactome
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Pathway
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Disease
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