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Last updated: 2016/10/16
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SPI1 monoclonal antibody (M01), clone 1A3

  • Catalog # : H00006688-M01
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against a partial recombinant SPI1.
  • Immunogen:
  • SPI1 (NP_003111, 1 a.a. ~ 120 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
  • Sequence:
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Isotype:
  • IgG1 Kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.

    QC Testing of H00006688-M01
    Western Blot detection against Immunogen (38.94 KDa) .
  • Storage Buffer:
  • In 1x PBS, pH 7.4
  • Storage Instruction:
  • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot analysis of SPI1 expression in transfected 293T cell line by SPI1 monoclonal antibody (M01), clone 1A3.

    Lane 1: SPI1 transfected lysate(31.083 KDa).
    Lane 2: Non-transfected lysate.
  • PDF DownloadProtocol Download
  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • Detection limit for recombinant GST tagged SPI1 is approximately 10ng/ml as a capture antibody.
  • PDF DownloadProtocol Download
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 6688
  • Gene Name:
  • SPI1
  • Gene Alias:
  • Gene Description:
  • spleen focus forming virus (SFFV) proviral integration oncogene spi1
  • Gene Summary:
  • This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • 31 kDa transforming protein,SPI-1 proto-oncogene,hematopoietic transcription factor PU.1
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