RPL13 monoclonal antibody (M01), clone 3F6
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant RPL13.
Immunogen
RPL13 (NP_000968, 112 a.a. ~ 211 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
ANVQRLKEYRSKLILFPRKPSAPKKGDSSAEELKLATQLTGPVMPVRNVYKKEKARVITEEEKNFKAFASLRMARANARLFGIRAKRAKEAAEQDVEKKK
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Rat (98)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
RPL13 monoclonal antibody (M01), clone 3F6 Western Blot analysis of RPL13 expression in HeLa ( Cat # L013V1 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged RPL13 is approximately 3ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to RPL13 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — RPL13
Entrez GeneID
6137GeneBank Accession#
NM_000977Protein Accession#
NP_000968Gene Name
RPL13
Gene Alias
BBC1, D16S444E, FLJ27453, FLJ27454, MGC117342, MGC71373
Gene Description
ribosomal protein L13
Omim ID
113703Gene Ontology
HyperlinkGene Summary
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L13E family of ribosomal proteins. It is located in the cytoplasm. This gene is expressed at significantly higher levels in benign breast lesions than in breast carcinomas. Transcript variants derived from alternative splicing and/or alternative polyadenylation exist; these variants encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq
Other Designations
60S ribosomal protein L13|OK/SW-cl.46|OTTHUMP00000165256|breast basic conserved protein 1
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Interactome
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Pathway
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