PITX1 monoclonal antibody (M02), clone 6D4
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant PITX1.
Immunogen
PITX1 (NP_002644, 225 a.a. ~ 313 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MPSSMGPGAVPGMPNSGLNNINNLTGSSLNSAMSPGACPYGTPASPYSVYRDTCNSSLASLRLKSKQHSSFGYGGLQGPASGLNACQYN
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (35.53 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
PITX1 monoclonal antibody (M02), clone 6D4 Western Blot analysis of PITX1 expression in A-431 ( Cat # L015V1 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PITX1 is approximately 0.03ng/ml as a capture antibody.ELISA
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Gene Info — PITX1
Entrez GeneID
5307GeneBank Accession#
NM_002653Protein Accession#
NP_002644Gene Name
PITX1
Gene Alias
BFT, POTX, PTX1
Gene Description
paired-like homeodomain 1
Omim ID
602149Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. Members of this family are involved in organ development and left-right asymmetry. This protein acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin. [provided by RefSeq
Other Designations
hindlimb expressed homeobox protein backfoot|paired-like homeodomain transcription factor 1|pituitary homeo box 1|pituitary otx-related factor
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Interactome
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Disease
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Publication Reference
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The c-Abl tyrosine kinase stabilizes Pitx1 in the apoptotic response to DNA damage.
Yamaguchi T, Miki Y, Yoshida K.
Apoptosis 2010 Aug; 15(8):927.
Application:WB-Ce, WB-Tr, Human, K-562, MCF-7, U-2 OS cells.
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The c-Abl tyrosine kinase stabilizes Pitx1 in the apoptotic response to DNA damage.
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