PFKP MaxPab mouse polyclonal antibody (B01)
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More Files
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Western Blot (Tissue lysate)
PFKP MaxPab polyclonal antibody. Western Blot analysis of PFKP expression in human pancreas.
Western Blot (Cell lysate)
PFKP MaxPab polyclonal antibody. Western Blot analysis of PFKP expression in Hela S3 NE.
Western Blot (Transfected lysate)
Western Blot analysis of PFKP expression in transfected 293T cell line (H00005214-T01) by PFKP MaxPab polyclonal antibody.
Lane 1: PFKP transfected lysate(86.24 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to PFKP on HeLa cell. [antibody concentration 10 ug/ml]
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Specification
Product Description
Mouse polyclonal antibody raised against a full-length human PFKP protein.
Immunogen
PFKP (NP_002618.1, 1 a.a. ~ 784 a.a) full-length human protein.
Sequence
MDADDSRAPKGSLRKFLEHLSGAGKAIGVLTSGGDAQGMNAAVRAVVRMGIYVGAKVYFIYEGYQGMVDGGSNIAEADWESVSSILQVGGTIIGSARCQAFRTREGRLKAACNLLQRGITNLCVIGGDGSLTGANLFRKEWSGLLEELARNGQIDKEAVQKYAYLNVVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAIMTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVFLPESPPEEGWEEQMCVKLSENRARKKRLNIIIVAEGAIDTQNKPITSEKIKELVVTQLGYDTRVTILGHVQRGGTPSAFDRILASRMGVEAVIALLEATPDTPACVVSLNGNHAVRLPLMECVQMTQDVQKAMDERRFQDAVRLRGRSFAGNLNTYKRLAIKLPDDQIPKTNCNVAVINVGAPAAGMNAAVRSAVRVGIADGHRMLAIYDGFDGFAKGQIKEIGWTDVGGWTGQGGSILGTKRVLPGKYLEEIATQMRTHSINALLIIGGFEAYLGLLELSAAREKHEEFCVPMVMVPATVSNNVPGSDFSIGADTALNTITDTCDRIKQSASGTKRRVFIIETMGGYCGYLANMGGLAAGADAAYIFEEPFDIRDLQSNVEHLTEKMKTTIQRGLVLRNESCSENYTTDFIYQLYSEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISARAMEWITAKLKEARGRGKKFTTDDSICVLGISKRNVIFQPVAELKKQTDFEHRIPKEQWWLKLRPLMKILAKYKASYDVSDSGQLEHVQPWSV
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (90); Rat (90)
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
No additive
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Note
For IHC and IF applications, antibody purification with Protein A will be needed prior to use.
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Applications
Western Blot (Tissue lysate)
PFKP MaxPab polyclonal antibody. Western Blot analysis of PFKP expression in human pancreas.Western Blot (Cell lysate)
PFKP MaxPab polyclonal antibody. Western Blot analysis of PFKP expression in Hela S3 NE.Western Blot (Transfected lysate)
Western Blot analysis of PFKP expression in transfected 293T cell line (H00005214-T01) by PFKP MaxPab polyclonal antibody.
Lane 1: PFKP transfected lysate(86.24 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to PFKP on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — PFKP
Entrez GeneID
5214GeneBank Accession#
NM_002627.3Protein Accession#
NP_002618.1Gene Name
PFKP
Gene Alias
FLJ40226, PFK-C, PFKF
Gene Description
phosphofructokinase, platelet
Omim ID
171840Gene Ontology
HyperlinkGene Summary
The PFKP gene encodes the platelet isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The PFKP gene, which maps to chromosome 10p, is also expressed in fibroblasts. See also the muscle (PFKM; MIM 610681) and liver (PFKL; MIM 171860) isoforms of phosphofructokinase, which map to chromosomes 12q13 and 21q22, respectively. Vora (1981) [PubMed 6451249] determined that full tetrameric phophofructokinase enzyme expressed in platelets can be composed of subunits P4, P3L, and P2L2.[supplied by OMIM
Other Designations
OTTHUMP00000018966|Phosphofructokinase, platelet type
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Fructose and mannose metabolism
- Galactose metabolism
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