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PFKP 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00005214-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-PFKP full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 85.6
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-PFKP antibody (H00005214-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00005214-T01
    PFKP transfected lysate.
    Western Blot
    QC Testing of H00005214-T01
    Lane 1: PFKP transfected lysate ( 85.6 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 5214
  • Gene Name:
  • PFKP
  • Gene Alias:
  • FLJ40226,PFK-C,PFKF
  • Gene Description:
  • phosphofructokinase, platelet
  • Gene Summary:
  • The PFKP gene encodes the platelet isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The PFKP gene, which maps to chromosome 10p, is also expressed in fibroblasts. See also the muscle (PFKM; MIM 610681) and liver (PFKL; MIM 171860) isoforms of phosphofructokinase, which map to chromosomes 12q13 and 21q22, respectively. Vora (1981) [PubMed 6451249] determined that full tetrameric phophofructokinase enzyme expressed in platelets can be composed of subunits P4, P3L, and P2L2.[supplied by OMIM
  • Other Designations:
  • OTTHUMP00000018966,Phosphofructokinase, platelet type
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