MBD1 MaxPab mouse polyclonal antibody (B01)
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Specification
Product Description
Mouse polyclonal antibody raised against a full-length human MBD1 protein.
Immunogen
MBD1 (NP_056671.2, 1 a.a. ~ 605 a.a) full-length human protein.
Sequence
MAEDWLDCPALGPGWKRREVFRKSGATCGRSDTYYQSPTGDRIRSKVELTRYLGPACDLTLFDFKQGILCYPAPKAHPVAVASKKRKKPSRPAKTRKRQVGPQSGEVRKEAPRDETKADTDTAPASFPAPGCCENCGISFSGDGTQRQRLKTLCKDCRAQRIAFNREQRMFKRVGCGECAACQVTEDCGACSTCLLQLPHDVASGLFCKCERRRCLRIVERSRGCGVCRGCQTQEDCGHCPICLRPPRPGLRRQWKCVQRRCLRGKHARRKGGCDSKMAARRRPGAQPLPPPPPSQSPEPTEPHPRALAPSPPAEFIYYCVDEDELQPYTNRRQNRKCGACAACLRRMDCGRCDFCCDKPKFGGSNQKRQKCRWRQCLQFAMKRLLPSVWSESEDGAGSPPPYRRRKRPSSARRHHLGPTLKPTLATRTAQPDHTQAPTKQEAGGGFVLPPPGTDLVFLREGASSPVQVPGPVAASTEALLQEAQCSGLSWVVALPQVKQEKADTQDEWTPGTAVLTSPVLVPGCPSKAVDPGLPSVKQEPPDPEEDKEENKDDSASKLAPEEEAGGAGTPVITEIFSLGGTRFRDTAVWLPRSKDLKKPGARKQ
Host
Mouse
Reactivity
Human
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
No additive
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Note
For IHC and IF applications, antibody purification with Protein A will be needed prior to use.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of MBD1 expression in transfected 293T cell line (H00004152-T01) by MBD1 MaxPab polyclonal antibody.
Lane 1: MBD1 transfected lysate(66.55 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to MBD1 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — MBD1
Entrez GeneID
4152GeneBank Accession#
NM_015846.2Protein Accession#
NP_056671.2Gene Name
MBD1
Gene Alias
CXXC3, PCM1, RFT
Gene Description
methyl-CpG binding domain protein 1
Omim ID
156535Gene Ontology
HyperlinkGene Summary
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters; in addition, variants with three CXXC domains also repress unmethylated promoter activity. MBD1 and MBD2 map very close to each other on chromosome 18q21. [provided by RefSeq
Other Designations
OTTHUMP00000163504|OTTHUMP00000163506|OTTHUMP00000163507|methyl-CpG binding domain protein 1 isoform PCM1|the regulator of fibroblast growth factor 2 (FGF-2) transcription
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Interactome
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Disease
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Publication Reference
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Ccdc13 is a novel human centriolar satellite protein required for ciliogenesis and genome stability.
Staples CJ, Myers KN, Beveridge RD, Patil AA, Howard AE, Barone G, Lee AJ, Swanton C, Howell M, Maslen S, Skehel JM, Boulton SJ, Collis SJ.
Journal of Cell Science 2014 Jul; 127(Pt 13):2910.
Application:IF, WB-Tr, Human, HeLa, RPE-1 cells.
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The centriolar satellite protein Cep131 is important for genome stability.
Staples CJ, Myers KN, Beveridge RD, Patil AA, Lee AJ, Swanton C, Howell M, Boulton SJ, Collis SJ.
Journal of Cell Science 2012 Oct; 125(Pt 20):4770.
Application:IP, WB-Tr, Human, HEK 293 cells.
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Ccdc13 is a novel human centriolar satellite protein required for ciliogenesis and genome stability.
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