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MBD1 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00004152-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-MBD1 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 66.66
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-MBD1 antibody (H00004152-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00004152-T01
    MBD1 transfected lysate.
    Western Blot
    QC Testing of H00004152-T01
    Lane 1: MBD1 transfected lysate ( 66.66 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 4152
  • Protein Accession#:
  • -
  • Gene Name:
  • MBD1
  • Gene Alias:
  • CXXC3,PCM1,RFT
  • Gene Description:
  • methyl-CpG binding domain protein 1
  • Gene Summary:
  • DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters; in addition, variants with three CXXC domains also repress unmethylated promoter activity. MBD1 and MBD2 map very close to each other on chromosome 18q21. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000163504,OTTHUMP00000163506,OTTHUMP00000163507,methyl-CpG binding domain protein 1 isoform PCM1,the regulator of fibroblast growth factor 2 (FGF-2) transcription
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