Western blot analysis of LCK over-expressed 293 cell line, cotransfected with LCK Validated Chimera RNAi ( Cat # H00003932-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with LCK monoclonal antibody (M01) clone 3F7-F5 (Cat # H00003932-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been described. [provided by RefSeq
T-lymphocyte specific protein tyrosine kinase p56lck,p56(LSTRA) protein-tyrosine kinase,protein tyrosine kinase,proto-oncogene tyrosine-protein kinase LCK